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Accumulation of reactive oxygen species (ROS) in embryos was assessed using 2’,7’-dihydrodichlorofluorescein diacetate (H2DCFDA) staining as previously described [41]. The green fluorescent ROS signal was observed under a fluorescence dissecting microscope (excitation at 485 nm, emission at 560 nm).
Copyright and License information: ©2017 Tu et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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