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Histology and hematoxylin-eosin-safranin (HES) staining
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Animals were sacrificed by vertebral dislocation. The eyes were rapidly enucleated and fixed in 4% paraformaldehyde (PFA) for 24 h at 4°C. Eye cups were embedded in paraffin and cut into 5 μm sagittal sections. The sections were deparaffinized, stained with HES, rinsed, and mounted in Moviol.
Copyright and License information: ©2017 Cubizolle et al
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