Cell proliferation assay

MP Mi-Young Park
MJ Min Hee Jung
EE Eun Young Eo
SK Seokjoong Kim
SL Sang Hoon Lee
YL Yeon Joo Lee
JP Jong Sun Park
YC Young Jae Cho
JC Jin Haeng Chung
CK Cheol Hyeon Kim
HY Ho Il Yoon
JL Jae Ho Lee
CL Choon-Taek Lee
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PC9, PC9-G, PC9-3-2, PC9-3-14, and A549 human lung cancer cell lines were seeded at 3 × 103 cells/well in 96-well plates and treated with gefitinib or AZD9291; 96 h later, cell proliferation was assessed using the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, Madison, WI, USA) according to the manufacturer's instructions. Three independent experiments were performed and used to determine mean half-maximal inhibitory concentration (IC50).

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