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Cellular viability following siRNA-transfection was assessed using the CellTiter-Blue® reagent (Promega, Madison, WI). Reduction of resazurin to resorufin was measured at various time points after the respective treatment using a plate reader (VICTOR™ X4, Perkin Elmer, Waltham, MA) per the manufacturer’s instructions. Cellular viability of siRNA-transfected cells was compared to cells transfected with a non-silencing control siRNA (siNEG). Detailed information can be found in Supplementary Table S1.
Copyright and License information: ©2017
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