Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Culture of J774.1 cells.

LG Laura A. Gonyar
MG Mary C. Gray
GC Gregory J. Christianson
BM Borna Mehrad
EH Erik L. Hewlett
request Request a Protocol
ask Ask a question
Favorite

Cells from the J774.1 (here J774) murine macrophage cell line were maintained at 37°C in Dulbecco's modified Eagle's medium with high glucose (Gibco) plus 10% heat-inactivated FBS in 5% CO2.

Copyright and License information:  ©2017 American Society for Microbiology.
All Rights Reserved.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A