Antibodies

In western blotting analysis, we used in this study the following primary antibodies: rabbit polyclonal anti-PARP (1:500) (Cell Signaling, Danvers, MA, USA; 9542), mouse monoclonal anti-p62 (1:1000) (BD Transduction Laboratories, New Jersey, USA; 610832), mouse monoclonal anti-HIF1α (1:500) (Novus Biologicals, Cambridge, UK; NB100–105). To study autophagy we used a rabbit polyclonal anti-LC3 (1:1000) (Novus Biologicals, Cambridge, UK; NB100–2220SS).

Mouse monoclonal anti-β-actin (1:10000) (Sigma Aldrich, St Louis. MO, USA; A5441) (1:10000) was used as loading control. The goat polyclonal anti-mouse IgG-Horseradish Peroxidase Santa Cruz Biotechnology Inc., Heidelberg, Germany; sc-2005) and anti-rabbit IgG-HRP (Santa Cruz Biotechnology Inc., Heidelberg, Germany; sc-2004) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS-0.1% Tween20 solution containing 3% of BSA (SERVA, Reno, NV, USA; 11943.03). For immunohistochemistry mouse monoclonal anti- Forkhead Box P3 (FoxP3) (Santa Cruz Biotechnology Inc., Heidelberg, Germany; sc-53876) or mouse monoclonal anti-CD8α (Santa Cruz Biotechnology Inc., Heidelberg, Germany; sc-7970) were used.