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Live-cell imaging and analysis
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Cells transfected with GFP-EB1 or GFP-EB1 mutants were imaged with a confocal microscope (Leica) at 37°C in at atmosphere containing 5% CO2. Time-lapse images of GFP-EB1 comets were captured at 2-sec intervals for 2 min as described previously [46]. Microtubule dynamics were analyzed using PlusTipTracker software [47, 48].
Copyright and License information: ©2017 Gao et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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