SNP genotyping was performed using an improved multiplex ligase detection reaction method (iMLDR, Genesky Bio-Tech Cod., Ltd., Shanghai, China) as previously described [44]. Randomly selected DNA samples from each genotype were sequenced using a ligation detection reaction method to validate the genotyping. The results of the ligation detection reaction analysis were consistent with the results of sequencing.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.