WST assay for cell viability

C4-2AT6 cells were plated in 96-well plates, allowed to attach for 24 h, and then treated with different concentrations of cabazitaxel or docetaxel and/or NAC. At the end of the incubation period, WST reagents were added to each well and incubated for 1 hr. In order to investigate the effect of ROS generation by docetaxel or cabazitaxel, C4-2AT6 cells were treated with docetaxel or cabazitaxel in the presence or absence of antioxidant NAC for 24h and evaluated cell survival. Cell viability was estimated by colorimetry, reading the color intensity in a plate reader at 570 nm. Cell viability was expressed as percent values in comparison with untreated cells.