Western Blot Analysis of Nrf2 Nuclear Localization and Phosphorylated MAPK.

AC Anyamanee Chaiprasongsuk
JL Jinaphat Lohakul
KS Kitipong Soontrapa
SS Somponnat Sampattavanich
PA Pravit Akarasereenont
UP Uraiwan Panich
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Total protein, cytosolic, and nuclear extracts were prepared, and immunoblotting assay was carried out to detect nuclear localization of Nrf2 and phosphorylation of MAPK (ERK, JNK, and p38), as previously described (Chaiprasongsuk et al., 2016).

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