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Main reagents and instruments

ZL Zhi-Yuan Li
YH Yan Huang
YY Yan-Ting Yang
DZ Dan Zhang
YZ Yan Zhao
JH Jue Hong
JL Jie Liu
LW Li-Jie Wu
CZ Cui-Hong Zhang
HW Huan-Gan Wu
JZ Ji Zhang
XM Xiao-Peng Ma
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The following reagents and instruments were used: 5% TNBS (Sigma, St Louis, MO, United States); phospho (p)MEK antibody (Cell Signaling Technology, Danvers, MA, United States); pERK antibody (Cell Signaling Technology); pCREB antibody (Cell Signaling Technology); MEK inhibitor U0126 (Calbiochem, Darmstadt, Germany); DMSO (Santa Cruz Biotechnology, Dallas, TX, United States); horseradish peroxidase goat anti-rabbit IgG (Beyotime, Shanghai, China); Von Frey filaments (Stoelting Co, Wood Dale, IL, United States); BME2410A thermal stimulator (Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China); electrophoresis system: Mini-PROTEAN 3 cell (Bio-Rad, Hercules, CA, United States); imaging system: ChemiDocTM (Bio-Rad, Hercules, CA, United States); PCR primers ABI 7300 (Thermo Fisher Scientific, Waltham, MA, United States).

Copyright and License information:  ©2017 The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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