The regions of interest (ROI) used in this study were 6.0 mm long by 0.35 mm deep segments that corresponded to the external threads or highly porous TM midsections of the implants. Within the ROI segments, the areas occupied by the metallic dental implants were subtracted to calculate the amount of new bone. All histomorphometric analyses were performed using Bioquant Osteo II image analysis software (OsteoMetrics, Inc., Atlanta, GA, USA) interfaced with an Olympus light/epifluorescent microscope and video subsystem.
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