Nuclear factor‐kappa B reporter assay

Nuclear factor‐kappa B (NF‐κB) promoter activity was determined using a reporter assay. The transient transfection of control or PALB2 overexpression MDA‐MB‐231 cells was performed using FuGENE6 Transfection Reagent (Roche Applied Sciences). NF‐κB reporter plasmid and the Renilla pRL‐TK internal control vector were used for each transfection experiment. The cells were incubated for 24 h and then harvested. The luciferase activity was measured using the Dual‐Luciferase Reporter Assay System (Promega, Madison, WI, USA). The luciferase activity was adjusted for transfection efficiency by normalizing firefly luciferase activity to the Renilla luciferase activity generated by pRL‐TK (Promega).