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Cells (1.4 × 104) were seeded in individual wells of 6-welled plates in regular culture medium (containing 0.5 μM PLX8394 for PBRTs). The next day, plates were washed and medium was replaced with medium supplemented with drugs of interest. Medium and drugs were changed every 2 days. After 9 days, cells were fixed in buffered formalin with 0.2% crystal violet. Plates were then scanned for quantitation via ImageJ.
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