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Complement-dependent cytotoxicity

HS Han Sun
LL Liqun Luo
BL Bachchu Lal
XM Xinrong Ma
LC Lieping Chen
CH Christine L. Hann
AF Amy M. Fulton
DL Daniel J. Leahy
JL John Laterra
ML Min Li
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CDC assay protocol was adapted from previous studies49,50. Cancer cell lines were treated with 0.4 mg ml−1 antibodies with or without murine serum complement (Rockland) for 2 h at 37 °C. Lyophilized complement sera from mouse was reconstituted in PBS and serially diluted 1:10 to 1:1,000. Heat inactivation was performed by 30-min incubation at 56 °C. Cell death was determined as LDH release.

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