2.7. In- vitro anti-glycation activity

In vitro antiglycation activity of DAE and DHE was examined by testing their ability to inhibit the fluorescence of BSA in accordance with a previous method.²⁹ The reaction mixture of BSA (10 mg/mL), 1.1 M fructose in 0.1 M phosphate buffered-saline (PBS), pH 7.4 containing 0.02% sodium azide with or without extract (DAE and DHE; dissolved in PBS; 50–500 μg/mL) was incubated in darkness at 37 °C for 1, 2, 3, and 4 weeks. AGE formation was measured by fluorescent intensity at an excitation wavelength 355 nm and emission wavelength 460 nm. Aminoguanidine (AG) was used as a positive control for this study. The percentage inhibition of AGE formation was determined by following formula: