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Nitric Oxide (NO) Assay by Griess Reaction
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AoECs were treated with MVsnmMLCK+/+, MVsnmMLCK-/-, LPS (10 μg/ml), or the combination LPS with MVs for 24 h. The culture medium was collected and mixed with Griess reagent (Sigma-Aldrich) and nitrate reductase. Sodium nitrite standards were used to normalize the assay.
Copyright and License information: ©2016 Gaceb, Vergori, Martinez and Andriantsitohaina.
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