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TTC staining and quantitative analysis of infarct volume
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One week post-transplantation, 5 rats in each group were anesthetized with 10% chloral hydrate. The brains were removed carefully and dissected into 2-mm thick coronal sections. The fresh brain slices were immersed in 0.5% solution of TTC (Sigma, USA) in PBS at 37°C for 30 min. The cross-sectional area of the infarction in each brain slice was calculated by Image J analysis software (NIH, USA). The total infarct volume for each brain was calculated by the summation of the infarcted area of all brain slices.
Copyright and License information: ©2017 Zhang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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