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BEP2D cells were harvested, suspended in LHC-8 medium, and seeded upon a base layer of soft agar at a density of 500 cells per 60mm dish. All experiments were conducted in triplicates. Dishes were maintained at 37°C in humidified incubator and were fed every 3 day. After 6 days, cells were showed by Giemsa staining, and the number of the colony formation was assessed by counting under microscope. CFE (colony form efficiency) (%) = (number of colonies)/(number of inoculation cells) × 100%.
Copyright and License information: ©2017 Li et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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