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A375 cells were treated with 3 uM vemurafenib, 10 nM decitabine or the combination vemurafenib/decitabine for 72 h. At the end of the incubation, apoptosis was assessed with the Annexin V Apoptosis Detection kit (sc-4252 AK, Santa Cruz Biotechnology, Inc) according to manufacturer’s instructions. Samples were processed with the Becton Dickinson LSR II flow cytometer. Analysis was performed with FlowJo software.
Copyright and License information: ©2017 Zakharia et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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