Establishment of Xenograft SCID Beige Mouse Model of Lung Cancer

A total 2.5 × 10⁶ A549.GFP. Luc cells were injected into the caudal vein of six to eight week female SCID Beige mice on day 0. On day 7, the tail vein injection was performed with 1 × 10⁷ non-transduced T cells or EphA2-specific T cells in 200 μL into the previous stereotactic tumor coordinates. The mice were anesthetized with rapid sequence inhalation isoflurane after 150 mg/kg D-luciferin was injected per mouse intraperitoneally. The mice were then photoed with serial noninvasive in vivo bioluminescence imaging system (Berthold LB983, Germany) in 10–15 minutes. The photons emitted from the luciferase-expressing tumor cells were quantified using NightOWLindiGo™ software (Berthold technologies, Germany). Animals were imaged on day 0 and day 7 after injections, then once a week thereafter. The mice were treated and housed according to protocols approved by the Medical Experimental Animal Care Commission of Zhejiang University.