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Proximity Ligation Assay

MW Michelle M. Williams
LL Linus Lee
DH Donna J. Hicks
MJ Meghan M. Joly
DE David Elion
BR Bushra Rahman
CM Courtney McKernan
VS Violeta Sanchez
JB Justin M. Balko
TS Thomas Stricker
ME Monica Valeria Estrada
RC Rebecca S. Cook
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Cells cultured in 96-well plates were fixed with methanol, stained with the Duolink (Sigma) PLA protocol according to manufacturer's directions using Mcl-1 (Santa Cruz, 1:25) and Bim (Santa Cruz, 1:25) antibodies, counterstained with Hoescht and scanned by ImageXpress Micro XL Automated Microscope. PLA fluorescent puncta and Hoescht-stained cells were enumerated using ImageJ software.

Copyright and License information:  ©2016-12-30

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