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ROS detection using carboxy-H2DCFDA
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In HTEC we used the Image-iT LIVE Green Reactive Oxygen Species Detection kit (Molecular probes, Eugene, OR, USA) according to the manufacturer’s recommendations and analyzed using a fluorescence microscope. In SKRC-52 cells, ROS was detected by incubating cells with 25mM carboxy-H2DFFDA (Life Technologies) at 37°C for 30 min before analyzing with Flow Cytometry and FACSDiva software (version 6.1.1; BD Biosciences).
Copyright and License information: ©2017 Buvall et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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