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2.4. Estradiol Determination in the Cell Culture Medium

LS Lennard Schröder
DR Dagmar Ulrike Richter
BP Birgit Piechulla
MC Mareike Chrobak
CK Christina Kuhn
SS Sandra Schulze
SA Sybille Abarzua
UJ Udo Jeschke
TW Tobias Weissenbacher
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For the determination of estradiol in the culture medium, a competitive enzyme immuno-assay (EIA) was applied as described previously [29]. The measurements were performed using an automated Immulite 2000 (DPC Biermann, Freiburg, Germany) hormone analyzer.

Copyright and License information:  ©2016 by the authors; licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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