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After intraperitoneal injection of 0.3% sodium pentobarbital anesthetic, the abdominal cavity was opened for spleen removal and measurement. Spleens were placed in 10% neutral formalin; fixed for 24 hours; and subsequently dehydrated, paraffin embedded, sectioned, H&E stained, and imaged using optical microscopy to record morphological structure characteristics.
Copyright and License information: ©2017 Cheng et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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