Antibodies were purchased from Invitrogen (REGγ), Proteintech (GSK-3β), Abcam (c-Myc, CyclinD1, β-actin, Ki-67) and Cell Signaling Technology (β-catenin). Fluorescent-labelled secondary antibodies were purchased from Jackson Immuno Research. Sorafenib was purchased from Selleckchem (Houston, TX, USA).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.