2.5. Effectiveness of the biosynthesized AgNPs as antimicrobial agent

The assessment of AgNPs as antimicrobial agent was explored using agar well diffusion assay [3]. The microorganisms (Bacillus mycoides, Escherichia coli, and Candida albicans) were seeded in the plates of nutrient agar, and then different concentrations (12.5, 25, 50, and 100%) of the biosynthesized AgNPs solution were added to the agar wells (15 mm) which were made previously using sterile cork borer. The inoculated plates were incubated for 3 h at 37 °C after that; the inhibition zone diameter was measured.