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To analyze the adhesion of T24 and RT4 cells to an endothelial monolayer, Fibronectin (Fn) was seeded into a 96-well plate. Cells formed a confluent monolayer within 2 days. Non-adherent cells were removed by washing with PBS. The adherent cells were quantified using the CCK-8 kit as described for the cell proliferation assay.
Copyright and License information: ©2017 Wang et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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