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Differentiation of naïve T helper cells
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Enteric naïve T helper cells were purified and cultured in an anti-CD3 (2μg/mL; 17A2; Biolegend) pre-coated plate. For Th2 differentiation, cells were stimulated in the presence of soluble anti-CD28 (1μg/mL; 37.51), recombinant mouse IL-2 (10ng/mL) (Peprotech), IL-4 (50ng/mL), anti-IFN-γ (1μg/mL; R4-6A2) and anti-IL-12 (1μg/mL; C17.8) (Biolegend). Polarised Th2 cells were analysed after 4 days of differentiation.
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