ATP content, as a measurement of mitochondrial function, was detected and quantified by CellTiter-Glo Luminescent Cell Viability Assay (Promega Corporation, Madison, WI). The luminescent signals released from the assay were measured with a Spark™ 10M multimode microplate reader (Tecan Trading AG, Männedorf, Switzerland). The cellular ATP content was calculated by comparing the luminescence of the treated cells with that of the control.
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