3.7. GC-MS Analysis of Alcohol Moieties

The amount of 0.1 mg isolated ester was dissolved in 0.2 mL of 2 M LiOH in methanol in a 2-mL vial. After the solution had been stirred for 2 h the product was extracted with heptane (3 × 300 μL). The organic upper layer was analyzed by GC-MS using a 30 m × 0.25 mm i.d., 0.25 μm Zebron ZB-50 GC capillary column (Phenomenex Inc., Torrance, CA, USA). The column oven was held at 80 °C for 1 min and then increased 20 °C per min to 290 °C, and maintained at 290 °C for 3 min. Injector and detector temperatures were 230 °C and 150 °C respectively. The carrier gas was helium and the flow rate was 1.0 mL/min. The long-chain alcohol moieties of 1 was identified based on comparison with an authentic sample (Sigma-Aldrich) and its EI-MS spectra by comparison with entries in the 2002 version of NIST/EPA/NIH Mass Spectral Library (NIST 02).