The patients in the case group (n = 140) were diagnosed with CRC as primary cancer and treated in the Proctologist Clinic and Colorectal Surgery Department of Liga Norte Riograndense Contra o Câncer (Rio Grande do Norte, Brazil). The control subjects were cancer-free blood donors (n = 140) from the hemotherapy service (Hemovida, Rio Grande do Norte, Brazil) and were recruited in 2014.
Both Peripheral blood samples and questionnaire answers were collected from all subjects. The clinicopathological data were obtained by consulting the patients’ clinical charts, intra-operative documentation, and pathology scoring. Furthermore, the CRC patients were followed up to 20 years by medical records.
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