3.1. Sampling Site and Plant Material

The plant material was collected in a mangrove area in the municipality of Pirambu, State of Sergipe (SE), Brazil (10°40′42″ S, 36°52′25″ W). The annual average temperature and rainfall in the region is 26 °C and 1650 mm, respectively, with the rainy season from March to August [42]. Stems (1 cm diameter) of the species Aristolochia trilobata were collected from plants in the vegetative growth stage between 9:00 a.m. and 12:00 p.m. from February to September 2014. The plant material was dried at 60 ± 1 °C for four days in a drying oven (Marconi MA 037) [43]. A voucher specimen was deposited in the herbarium of the Federal University of Sergipe (Universidade Federal de Sergipe—UFS), registration no. ASE 35.723. The herbarium is located in the Department of Biology (Departamento de Biologia), Biological Sciences and Health Center (Centro de Ciências Biológicas e da Saúde—CCBS), municipality of São Cristóvão, SE, Brazil, zip code 49100-000.