Cell viability assays

Cell viability was examined using the CellTiter-Glo Assay kit (Promega Corporation), according to the manufacturer's protocol. Cells were plated onto a 96-well plate at a density of 2,000 cells/well. At 24, 48, 72 and 96 h, CellTiter-Glo reagent was added to the culture medium, and plates were agitated at room temperature for 10 min. The half-maximal inhibitory concentration was the concentration of GANT61 required for 50% inhibition of the cell viability in the curve. The luminescent signal was determined using a GloMax absorbance plate reader at a wavelength of 560 nm. Each individual experiment was performed at least three times independently.