Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
For the apoptosis assay, cells were labeled using Annexin V-FITC (BD Biosciences, San Jose, CA, USA). The mOrange-positive stromal cells were excluded, and the percentage of apoptotic AML cells (mOrange-negative, FITC-positive) was determined as described [4]. Data were acquired on the LSRII (BD) and analyzed with Diva (BD Biosciences). The percentage of spontaneous apoptosis was subtracted from the drug-treated samples to yield the percentage of apoptosis attributed to drug treatment, as described [15].
Copyright and License information: ©2017 Long et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
