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Female NCR-NU mice (Taconic, six–seven weeks old) were maintained and treated under pathogen-free conditions. MDA-MB-231 cells (1 × 106 cells/mouse) were injected subcutaneously to female NCR-NU mice. The injected mice were maintained under standard humane conditions. Tumor volume (V) was measured with calipers periodically and calculated by using formula V = 0.5 × LW2 (L, Length; and W, width). Mice were euthanized after 30–40 days. Tumors were removed, weighed and photographed.
Copyright and License information: ©2017 Li et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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