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Animals were killed under anaesthesia (400 mg/kg bodyweight ketamine, 40 mg/kg bodyweight xylazine) after one or four weeks post-transplantation. Samples of liver tissue and pancreatic tissue were either frozen or perfusion-fixed and embedded in paraffin or fixed in glutaraldehyde and embedded in glycidether. Paraffin slides of 1-2 μm thickness were serially cut and stained with H&E and the periodic acid Schiff (PAS) reaction.
Copyright and License information: ©2017 Ribback et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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