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MRI was performed in vivo at 2 days post injury in anesthetized mice to determine the lesion volume using a horizontal 9.4 T Oxford NMR 400 magnet (Oxford instrument PLC, Abington, UK) as previously described.40 Multi-slice T2-weighted images were acquired: echo time/repetition time of 40 ms/3000 ms, matrix size 128 × 256, field of view 19.2 × 19.2 mm2, slice thickness 0.8 mm and number of slices 12. Images were then analyzed using Aedes software (Kuopio, Finland) under MatLab program (Math-works, Natick, USA).
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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