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Transfection of pEYFP-ABR, pcDNA3.1-E2F1 and the according control vectors as well as ABR siRNA, CEBPα siRNA or control siRNA in U937 cells was performed via electroporation with a specific Nucleofactor Kit according to the manufacturer’s instructions (Lonza). 2 µg of DNA plasmid or 800 nM siRNA were used for each transfection. The pEYFP-ABR construct, as well as the empty vector pEYFP, were kindly provided by Professor Dr. N. Heisterkamp [23]. The siRNA for ABR and C/EBPα were purchased from Invitrogen. The siRNA sequences are provided in Supplementary Table 2.
Copyright and License information: ©2017 Namasu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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