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BxPC-3 and PANC-1 cells were trypsinized and seeded in a 24-well plate at a concentration of 8×104 cells per well. After overnight incubation, the cell monolayers were scratched using a plastic tip across the plate. The wells were washed three times with PBS to make sure that no suspending cells existed in the wound areas. Subsequently, the cells were incubated in low-serum (2%) medium with treatments and the wound images were taken as 0 h. After 24 h, 48 h, and 72 h, wound healing pictures were taken.
Copyright and License information: ©2017 Zhou et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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