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Streptomyces coelicolor germination assay.

KG Kirk J. Grubbs
RB Rachel M. Bleich
KM Kevin C. Santa Maria
SA Scott E. Allen
SF Sherif Farag
ES Elizabeth A. Shank
AB Albert A. Bowers
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Absorbance measurements for germination were done by diluting spores 1:10 in GYM medium in a 96-well plate with 50 µM compound in DMSO. The plate was shaken at 30°C and 300 rpm, while absorbance measurements were taken on an Infinite m200 Pro Tecan. The assay was run in triplicate.

Copyright and License information:  ©2017 Grubbs et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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