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Mechanical allodynia was assessed by examining the paw withdrawal mechanical threshold (PWMT) in response to a calibrated series of Von Frey hairs (29). Each filament was applied five times, each application lasted for 2 sec, and there was a 30-sec interval between each application. A positive response to a filament was indicated by the withdrawal of a hind paw upon application of a particular hair for at least 3 out of 5 consecutive applications. The PWT was defined as the smallest value of the hair force in grams that elicited positive responses.
Copyright and License information: ©2017 Ji et al.
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