The cyclooxygenase (COX)-2/soluble epoxide hydrolase (sEH) dual inhibitor, PTUPB, was synthesized by Dr. Sung Hee Hwang as described [18, 47]. The recombinant human EGF (hEGF) (#8916) and primary antibodies against β-actin (#3700), CDK2 (#2546), p27Kip1 (#3686), Cyclin D3 (#2936), c-Myc (#13987), Cyclin D1 (#2978), p21Waf1/Cip1 (#2947), CDK4 (#12790), CDK6 (#3136), EGFR (#4267), p-EGFRTyr1068 (#3777), p-EGFRTyr1173 (#4407), AKT (#2920), p-AKTSer473 (#4060), ERK1/2 (#4695), p-ERK1/2Thr202/Tyr204 (#4377), GRB2 (#3972), BMI1 (#6964), ZEB1 (#3396) and SOX2 (#3579) were all purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against HMMR (#GTX121502) was purchased from GeneTex (Irvine, CA, USA).
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