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Affinity Purification of Anti-Peptide Antibodies
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The peptides (5 mg peptide per gram of dried Sepharose powder) were coupled to Sepharose 4B (Pharmacia) following the manufacturer's instructions. Sera diluted in PBS were applied to the columns. Eluted Igs were dialyzed against PBS. The purity of the preparations was checked by SDS-PAGE followed by silver staining.
Copyright and License information: ©2017 Puccetti et al
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