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Serum Stability Determination
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In vitro stability of DFO-mAb was assessed in serum for up to 3 days. For the serum stability studies, 50 μCi 89Zr- DFO-mAb was added to 500 μL of 100% fetal bovine serum and the mixture was incubated at 37 °C. Aliquots were reserved at different time points: 1 h, 4 h, 24 h, 75 h, and 5 days and assessed by iTLC. Activity within the serum samples was resolved by iTLC, and each experiment was carried out in triplicate.
Copyright and License information: ©2017 American Chemical Society
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