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All experimental procedures were performed in accordance with the National Institutes of Health guide for the care and use of laboratory animals. In all, 1 × 107 cells were resuspended in 100 μl PBS, and injected subcutaneously into the lateral flanks of immunodeficient mice. Tumor volumes were measured weakly, with calculation using the equation: V (cm3) = width2 (cm2) × length (cm)/2. After 4 weeks, tumors were harvested for immunostaining after tumor implantation.
Copyright and License information: ©2017 Song et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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