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Cells were treated with 30 PFU/cell Reolysin for 48 h and processed for electron microscopy. Sections were cut in an LKB Ultracut microtome (Leica, Deerfield, IL), stained with uranyl acetate and lead citrate, and examined in a JEM 1230 transmission electron microscope (JEOL, USA, Inc., Peabody, MA). The percentage of reovirus positive cells were determined by counting 500 cells in random fields using an electron microscope. The fraction of cytoplasm containing containing reoviral particles was quantified using ImageJ software.
Copyright and License information: ©2017 Carew et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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