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Enzyme-linked immunosorbent assay (ELISA)
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PBMo-derived macrophages, TAM-like macrophages and ESCC cell lines were cultured in 24-well plates (1 × 105 cells/well) with RPMI-1640 10% FBS and 1% antibiotic-antimycotic. After 48 h, the CXCL8 levels in the cell culture supernatants were determined by the Quantikine ELISA Human IL-8 immunoassay (R&D Systems) according to the manufacturer's instructions. The optical density of each well was determined by the Infinite 200 PRO microplate reader at 492 nm. The CXCL8 concentration of each sample was calculated using a standard curve and the measured absorbance.
Copyright and License information: ©2017 Hosono et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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