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Three mice in each of the two experimental groups, osteophyte and cancellous bone, were killed at three and six weeks following transplantation, for histological analysis. The whole calvaria were fixed in 4% paraformaldehyde, decalcified with 0.5 M ethylenediaminetetraacetic acid and embedded in paraffin wax. Coronal sections (5 μm thick) were stained with Safranin O, fast green and Weigert’s iron haematoxylin, and examined by light microscopy. Concurrently, consecutive slices were stained with haematoxylin and eosin (H&E).
Copyright and License information: ©2017 Okazaki et al.
This is an open-access article distributed under the terms of the Creative Commons Attributions licence (CC-BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited.
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