Transcriptomic analysis by RNA-seq

Trancriptomic analysis of the silique walls of Col-0 and BnLATE transgenic Arabidopsis line L8 was executed using RNA-Seq with an Illumina HiSeqTM 2000 platform (Sangon Biotech, Shanghai, China). RNA-Seq data were processed as previously described [29]. To identify differentially expressed genes (DEGs), a previously described method was used [30]. Finally, FDR ≤ 0.05 and an absolute value of Log₂ ratio ≥ 1 were selected as the significance threshold of differential gene expression in the siliques of wild type Col-0 and transgenic line L8. Gene ontology and pathway mapping analysis of the identified DEGs were performed using MapMan and the KEGG database.